ABSTRACT
Ginko biloba extract is an active compound with broad biological function.This review summarizes the immuomodulatory effect of Ginko biloba extract on immune organ development, monocyte macrophage, natural killer cell, humoral immunity, cellular immunity, cytokines, red cell immunity and mucosal immunity.
ABSTRACT
OBJECTIVE:To draw a comparison among four extraction methods of total DNA from Corydalis saxicola in order to get high quality DNA sample.METHODS:The total DNA was extracted by palmityl trimethyl ammonium bromide method,enzyme-added detergent method,guanidinium isothiocyanate method,inertmacromolecular adsorptive column method,respectively.The purity(defined as RA260/A280)and the yield of the total DNA were determined.RESULTS:The RA260/A280 values of the DNA samples extracted by 4 methods were 1.832 9,1.699 2,1.812 2 and 1.813 6,respectively;and the extraction rates were 140.20 ?g?g-1,69.56 ?g?g-1,132.50 ?g?g-1 and 143.12 ?g?g-1,respectively.CONCLUSION:DNA samples extracted in 4 methods were all of high quality.The yield of DNA sample in enzyme-added extraction method was lower,while the guanidinium isothiocyanate method and the adsorption column method were rapid and simple and they are suitable for the test of samples of small size.
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OBJECTIVE: To establish an HPLC method for the determination of Baicalin in Tanreqing injection.METHODS: The determination was performed by HPLC using an CLC-ODS C18 column(150 mm?6.0 mm,5 ?m),with mobile phase consisted of methanol-water(47∶53) at a flow rate of 0.5 mL?min-1 and a detection wavelength of 280 nm.RESULTS: The calibration curve for Baicalin was linear in the concentration range of 2.53～12.65 ?g?mL-1(r=0.999 8).The average recovery was 99.61%(RSD=0.35%,n=9).CONCLUSION: The method is simple,rapid and applicable for the quality control of Tanreqing injection.
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OBJECTIVE:To establish HPLC method for the determination the centent of tanshinone Ⅱ_A in danshen shuxin capsule.METHODS:The determination was performed on Shim-pack CLC-ODS column,the mobile phase consisted of methanol-water(15∶5),the detection wavelength of270nm,column temperature at40℃,a flow rate of1.5ml/min and.RE_ SULTS:The calibration curve of tanshinone Ⅱ_A is linear when its concentration range was with in 0.05125～0.25625?g/ml(r=0.9996,n=5),the average recovery was 99.05%(RSD=0.92%).CONCLUSION:This method is convenient in operation,precise,reliable in results,and it can be used as quality control for danshen shuxin capsule.